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To support sustainable construction, the application of recycled coarse aggregate was used in the experiment for making porous concrete. As the quality of recycled aggregate is lower than natural coarse aggregate due to large pores and higher water absorption, ureolytic bacteria was utilized to produce calcium carbonate precipitation to fill the pores and finally enhanced the quality of recycled coarse aggregate. S17 and R20 ureolytic bacteria were used to produce calcium carbonate precipitation. The result show that the tensile splitting strength of recycled aggregate porous concrete improved compared to recycled aggregate porous concrete without ureolytic bacteria. Moreover, when using in porous concrete, R20 provide higher tensile splitting strength compared to natural aggregate porous concrete.