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This research focuses on analyzing the significant effect of the voltage and flow rate of oxygen fed into the ozone generator on the concentration of ozone produced into the sputum chamber. In designing the sputum chamber, it is necessary to test the things that are likely to affect the results of the ozone content in the room and what kind of effect it has. The first is related to the impact of the voltage value on the ozone content, where the independent variable is the amount of voltage which in the test is worth 3370V and 4316V, the controlled variable is the value of the oxygen flow rate is sets at 1L/minute for 30 minutes. The dependent variable is the value of the ozone concentration. The results obtained with a voltage of 3370V the value of the ozone concentration formed was 0.056mgO₃/L, while at a voltage of 4316V, the ozone concentration value included was 0.088mgO₃/L. In the second test with the independent variable in the form of oxygen flow rate, which in this test is 1L/minute and 3L/minute, the controlled variable is the voltage that enters the ozone generator at 4324V with a test time of 30 minutes, and the dependent variable is the value of ozone concentration—formed in the chamber. The second test result is that at an oxygen flow rate of 1L/min, the concentration of ozone formed is 0.088mgO₃/L, while at a rate of 3L/min, the concentration is 0.026mgO₃/L. From these two tests, it can be concluded that the greater the value of the voltage fed into the ozone generator, the concentration of ozone produced will also increase (directly proportional). In contrast, if the value of the oxygen flow rate is more excellent, the value of the ozone concentration in the room will decrease (inversely proportional to the value of the oxygen flow rate).